简介：Thedynamicmicrocosmswereusedtoevaluatetheeffectofoilspillsonmicrobialecologicalsysteminmarinesedimentandtheenhancementofnutrientontheoilremoval.ThefunctionandstructureofmicrobialcommunitycausedbytheoilpollutionandphosphatedosageweresimultaneouslymonitoredbydehydrogenaseactivityassayandPCR-denaturinggradientgelelectrophoresis(DGGE)techniques.Theresultsindicatedthattheamountoftotalbacteriainalldynamicmicrocosmsdeclinedrapidlywithincubationtime.Thenumberofpetroleum-degradingbacteriaandtheactivityofsedimentdehydrogenaseweregraduallyenhancedbypetroleumintheoil-treatedmicrocosms,whiletheybothshowednoobviousresponsetophosphatedosage.Incomparison,phosphatespikedheterotrophicbacteriaandtheyshowedasignificantincreaseinamount.DGGEprofilesindicatedthatpetroleumdosagegreatlychangedcommunitystructure,andthebacteriabelongedtoclassDeltaproteobacteria,andphylaBacteroidetesandChlorobiwereenriched.Thisstudydemonstratedthatpetroleuminputgreatlyimpactedthemicrobialcommunitystructureandconsequentlythemarinesedimentpetroleum-degradingactivitywasenhanced.Phosphatedosagewouldmultiplyheterotrophicbacteriabutnotsignificantlyenhancethepetroleumdegradation.

简介：Lornoxicam(6-chloro-4-hydroxy-2-methyl-N-2-pyridyl-2H-thieno-[2,3-e]-1,2-thiazine-3-carboxamide-1,1-dioxide)ispersistasanon-steroidalanti-inflammatorydrugoftheoxicamclasswithanalgesic,anti-inflammatoryandantipyreticproperties.Afast,accurateandsensitivechromatographicmethodforestimationofLornoxicamwasdevelopedasnoofficialmethodavailablefordetection.ThechromatographicseparationemploysisocraticelutionbyutilizinganinertsilODS-C18,250mm×4.6mm,5μmcolumns.Mobilephaseconsistingofsolvent(40mLacetonitrileand60mL0.1Mphosphatebuffer(pH6.8wasadjustedwithtriethylamine))endowedataflowrateof1.0mL/min.Theanalytewasdetectedandquantifiedat290nmusingUVdetector.ThemethodwasvalidatedaccordingtoICHguidelines,illustratingtobeaccurate(recovery99.08%-101.13%)andprecise(intraday(0.27-1.32)andinterday(0.59-1.59))withinthecorrespondinglinearrange(10-60μg/mL)withr20.9992.