简介:AbstractObjective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen (2H/1H) exchange at the metabolic level upon exposure to heavy water (2H2O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water. The treated mouse was fed with 2H2O (80%, v/v) and the control mouse fed with standard drinking water (H2O) over next 13 days. Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1, 2, 3, 10, 12, and 13. Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University (approval No. 20161117163322) on October 29, 2021.Results:We observed a replacement of 1H by 2H in 52 mass spectral features (60 2H/1H isotopologue pairs) for the mouse fed with 2H2O, but not for the control mouse. These included pyruvic acid and lactic acid, lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid, propionic acid, butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo2H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use 2H tracing to extend current metabolic studies, especially those with a focus on anaerobic glycolysis, lysine methylation and gut microbiome via monitoring of short-chain fatty acids.
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简介:AbstractBackground:The complete mesogastrium excision (CME) based on D2 radical gastrectomy is believed to significantly reduce the local-regional recurrence compared with D2 radical gastrectomy in advanced gastric cancer, and it is widely used in China. This study aimed to explore whether D2 + CME is superior to D2 on surgical outcomes during gastrectomy from Chinese data.Methods:Feasible studies comparing the D2 + CME (D2 + CME group) and D2 (D2 group) published up to March 2020 are searched from electronic databases. The data showing surgical and complication outcomes are extracted to be pooled and analyzed.Results:Fourteen records including 1352 patients were included. The D2 + CME group had a shorter mean operative time (weighted mean difference [WMD] = -16.72 min, 95% confidence interval [CI]: -26.56 to - 6.87 min, P < 0.001), lower mean blood loss (WMD = -39.08 mL, 95% CI: -49.94 to -28.21 mL, P < 0.001), higher mean number of retrieved lymph nodes (WMD = 2.13, 95% CI: 0.58-3.67, P = 0.007), shorter time to first flatus (WMD =-0.31 d, 95% CI: -0.53 to - 0.10 d, P = 0.005), and postoperative hospital days (WMD =-1.09, 95% CI: -1.92 to -0.25, P = 0.010) than the D2 group. Subgroup analysis suggested that the advantages from the D2 + CME group were obvious in traditional open radical gastrectomy, proximal gastrectomy, and distal gastrectomy compared with D2 group. The evaluations of post-operative complications showed that the patients who underwent D2 + CME had a lower incidence of post-operative complications than the patients who underwent D2 surgery alone (relative risk [RR] = 0.65, 95% CI: 0.45-0.87, P = 0.003). The D2 radical gastrectomy plus CME improved 3-year overall survival (OS) (RR = 1.16, 95% CI: 1.02-1.32, P = 0.020) and lowered the local recurrence rate (RR = 0.51, 95% CI: 0.28-0.94, P = 0.030). The patients undergoing laparoscopic surgery or total gastrectomy had more significant advantages compared between D2 + CME and D2 groups in 3-year OS.Conclusion:The data from China show that D2 radical gastrectomy plus CME are reliable procedures and safety compared to D2 radical gastrectomy with faster recovery, lower risk, and better prognosis.
简介:摘要:目的:分析"H2H"营养管理模式对鼻咽癌放疗患者临床结局的影响。方法:选取我院鼻咽癌放疗患者共83例,按照电脑乱序分组的方法将其分为实验A组与实验B组,其中实验A组41例,实验B组42例。传统的护理方法应用于实验A组患者,实验B组患者则在传统护理的基础上实施“H2H”营养管理模式。结果:相较于实验A组,实验B组患者口腔黏膜急性放射性反应与放疗后的缓解状况更优,生活质量评分更高,P<0.05。结论:对鼻咽癌放疗患者实施传统护理与“H2H”营养管理模式联合的护理方式能够增强患者的营养支持,改善其口腔粘膜急性放射性反应状况,提高治疗有效率,优化生活质量,安全性高,值得推广。
简介:摘要目的在小鼠中筛选和确定尼帕病毒(Nipah virus,NiV)融合糖蛋白(fusion glycoprotein,F)和附着糖蛋白(attachment glycoprotein,G)特异性的H-2d限制性T细胞表位。方法本研究基于NiV F和G蛋白全序列设计合成多肽库(单肽长为15个氨基酸,前后肽重复10个氨基酸),使用表达NiV F和G蛋白的DNA疫苗肌肉注射免疫BALB/c小鼠,通过矩阵设计将F和G抗原全序列肽库分别混合成肽池,取免疫后小鼠脾细胞进行IFN-γ ELISPOT检测,确定肽库中特异反应的优势表位肽。结果F肽库筛选到12条特异性优势肽,第56条多肽产生的反应最强;G肽库筛选到4条特异性优势肽,第72条多肽产生的反应最强。结论本研究使用IFN-γ ELISPOT方法筛选和确定了12条NiV F抗原特异性和4条NiV G抗原特异性H-2d限制性优势T细胞表位,为NiV免疫学与疫苗研究提供了参考。
简介:摘要:在硫回收装置中,将H2S/SO2比值当成是重要的工艺控制参数,需通过准确测量气体浓度反映装置节能效率,为加强环境污染控制提供科学依据。结合某石化公司案例展开分析,可知在克劳斯硫磺回收装置运行期间,配备紫外线气体分析仪对H2S/SO2比值进行连续测量,通过对仪器运用问题展开分析,提出使用建议,能够做到精准监测尾气中的污染气体含量。
简介:摘要目的探讨"H2H"营养管理模式对食管癌放疗患者营养状况、生活质量及并发症的影响。方法按随机数字表法将2019年6月至2020年6月在华中科技大学同济医学院附属同济医院进行放射治疗的食管癌患者82例分为观察组和对照组各41例。对照组给予常规护理干预,观察组实施"H2H"营养管理模式。比较两组患者治疗效果、并发症发生情况、护理满意度及干预前后的营养状况和生活质量。结果观察组患者缓解率明显优于对照组;干预后,两组PG-SGA分级比较均明显改善,且观察组改善效果优于对照组;干预后,观察组与对照组生活质量评分比较均明显升高,且观察组优于对照组;观察组患者并发生症总发生率明显低于对照组,差异均有统计学意义(均P<0.05)。结论"H2H"营养管理模式能够有效增加食管癌放疗患者的治疗效果,并能够明显改善患者的营养状况,提高生活质量的同时减少并发症。
简介:摘要目的探讨3D腹腔镜精索静脉精准结扎术治疗成人原发性临床型精索静脉曲张的临床价值。方法选取2016年3月至2019年1月符合条件的241例原发性临床型精索静脉曲张患者,其中123例行2D腹腔镜手术(2D组),118例行3D腹腔镜手术(3D组)。对两组患者的手术时间、精索内动脉识别率、术后精液质量改善率、复发率、并发症发生率进行对比分析。结果2D组的手术时间长于3D组,差异有统计学意义(P<0.05);3D组的精索内动脉识别率显著高于2D组,差异有统计学意义(90.7% vs.74.8%,P<0.05);在并发症发生率方面,3D组的睾丸鞘膜积液发生率显著低于2D组(P<0.05),两组的附睾炎、阴囊水肿、睾丸萎缩发生率比较,差异均无统计学意义(均P>0.05);术后随访6~12个月,3D组的精液质量改善率明显优于2D组(87.3% vs.74.0%,P<0.05);两组患者的复发率比较,差异无统计学意义(8.1% vs. 5.9%,P>0.05)。结论与2D腹腔镜相比,3D腹腔镜精索静脉精准结扎术手术时间更短,并发症发生率更低,精液质量改善率更高,有条件的医院可将其作为腹腔镜治疗精索静脉曲张的首选方法。
简介:摘要:随着我国完成建设进入运营期PPP项目数量的增多,PPP项目的绩效评价和中期评估也逐渐引起项目各参与主体的重视。本文将结合PPP项目特点利用5W2H方法对PPP项目中期评估目的、评估流程等核心问题进行系统性梳理,加强对PPP项目中期评估应用效果理解,切实发挥出PPP项目中期评估的作用,确保项目后期运营的稳定性。
简介:摘要目的对2例短肋多指综合征3型(short rib polydactyly syndrome type Ⅲ,SRPSⅢ)的胎儿进行产前诊断,明确其发病的分子遗传学病因。方法应用二代测序技术(next generation sequencing,NGS)对2015年8月和2020年6月于郑州大学第一附属医院就诊的2个患病胎儿(先证者)进行遗传性骨病226个相关致病基因检测。发现可疑致病位点后,对家系成员进行Sanger双向测序验证分析。确定致病变异后,对家系1的高危胎儿进行产前诊断。结果2个SRPS Ⅲ家系均发现了DYNC2H1基因变异,其中家系1先证者携带DYNC2H1基因c.5881A>G(p.Lys1961Glu)纯合变异,父母分别为携带者;家系2先证者DYNC2H1基因存在c.10606C>T(p.Arg3536*)和c.8954T>G(p.Val2985Gly)复合杂合变异,分别来自父亲和母亲。2个家系均符合常染色体隐性遗传,DYNC2H1基因c.5881A>G(p.Lys1961Glu)、c.8954T>G(p.Val2985Gly)为首次报道的疑似致病性变异。产前诊断结果显示家系1胎儿(Ⅱ-7)未见DYNC2H1基因c.5881A>G(p.Lys1961Glu)变异,孕妇选择继续妊娠,分娩后取脐带血行基因检测,结果与产前基因诊断结果一致。结论DYNC2H1基因变异是这2个家系的致病原因。
简介:摘要目的对比分析基于压缩感知(compressed sensing,CS)技术的3D液体衰减反转恢复(fluid-attenuated inversion-recovery,FLAIR)序列与常规2D-FLAIR序列对脑白质病变的成像质量。材料与方法前瞻性纳入首都医科大学附属北京天坛医院40例同时行头部CS 3D-FLAIR序列和2D-FLAIR序列扫描的脑白质病患者。客观分析图像的信噪比(signal-to-noise ratio,SNR)和对比噪声比(contrast noise ratio,CNR),并由2名经验丰富的诊断医师对图像整体质量和脑白质病灶个数进行评估。利用Wilcoxon秩和检验进行统计分析。结果CS技术可以将3D-FLAIR序列的采集时间缩短到与2D-FLAIR序列相当。CS 3D-FLAIR序列和2D-FLAIR序列图像的SNR和CNR差异无统计学意义(Z=-1.18,P=0.24;Z=-1.92,P=0.14)。CS 3D-FLAIR序列的图像整体质量评分和显示脑白质病灶个数显著优于2D-FLAIR序列(Z=-3.99,P<0.001;Z=-3.75,P=0.006)。CS 3D-FLAIR序列对第四脑室层面的搏动伪影具有抑制作用。结论扫描时长相当的情况下,CS 3D-FLAIR序列图像整体质量好,可抑制搏动伪影,比2D-FLAIR序列更利于脑白质病变的检出,建议临床推广应用。
简介:摘要:目的:研讨基于H2H营养管理模式的延续护理对I型糖尿病患儿血糖及酮症酸中毒发生率的影响。方法:选择来2020年1月-2022年1月我院就医的I型糖尿病患儿作为本次医学研究对象,从中随机选择42例患儿,并再次随机分配为对照组与观察组,单组21例,观察组实施基于H2H营养管理模式的延续护理,对照组实施常规延续护理,对比两组血糖控制情况及酮症酸中毒发生率。结果:观察组患儿血糖控制水平显著高于对照组,P<0.05;酮症酸中毒发生率对比,观察组显著高于对照组,P<0.05。结论:针对I型糖尿病患儿,实施基于H2H营养管理模式的延续护理,患儿的血糖控制效果较为理想,利于患儿早日恢复健康,平稳控制血糖,减轻患儿生活负担,可以在临床护理中推广应用。