简介：AIM:Toexaminethemechanismofthedevelopmentofpseudoexfoliation(PSX)syndromeviabothcytokineformationandendothelialvasorelaxingandgrowthfactorsthatwillprovideusnewtherapeuticinsightsforthetreatment.METHODS:Thisisacrosssectionalstudyincludedtwogroups;Group1:controlpatientswithnuclearcataract(n=20,aged51-80years).Group2:PSXpatientswithnuclearcataract(n=18,aged50-90years).Patientswithotherophthalmicproblemsandsystemicdiseaseswereexcluded.Vascularendothelialgrowthfactor(VEGF),interleukin-6(IL-6)andinterleukin-1β(IL-1β)andnitrotyrosinelevelsweredeterminedthroughserumsamplesbyEnzyme-linkedimmunosorbentassay(ELISA)method.Nitrite-nitratelevelsweremeasuredwithphotometricendpointdetermination.RESULTS:Therewerenosignificantdifferencesbetweenthegroupsintermsofage,VEGF,IL-1β,nitrite-nitrateandnitrotyrosine.ThesignificantresultswerethemeanIL-6levelsthatwerehigherinPSXgroup2(37.68±29.52pg/mL)comparedtothatincontrolgroup1(15.32±10.08pg/mL)(P<0.001).CONCLUSION:SeveralinteractingandextendingbiochemicalpathwaysmayleadtothepromotionofVEGFandIL-6expressions.IL-6whichistheonlyalteredmarkerinourstudymayindirectlycauseanincreaseofvascularpermeabilityandneovascularization.WesuggestinflammationasafactorthatcanbeinvolvedinetiopathogenesisofPSX.

简介：AIM:Todeterminethehistopathologicalchangesofrifampicinappliedintravitreallyonretinalganglioncellsbymeansofstereologicalandhistopathologicalmethods.METHODS:Forthisstudytwenty-fourNewZealandadultrabbitsweredividedintofourgroups(n=6foreachgroup).50μg/0.1mL(group1),100μg/0.1mL(group2),150μg/0.1mL(group3)and200μg/0.1mL(group4),rifampicinwereinjectedintothevitreousoftherighteyesofanimals,theirlefteyeswereusedascontrol(group5).Afterthe28thdayofapplication,animalswereanesthetisedwithxylazine(8mg/kg,IM)andthentheireyeswereenucleatedimmediately.Patternsweretakenawayandeyeswerepreparedforbothstereologicalandelectromicroscopicobservation.RESULTS:Dependingonthehighdoseofrifampicin,somehistopathologicalchangessuchascytoplasmicdilatationanddamagedmembranewereobservedontheelectromicroscopiclevel.Usingquantitativeexamination,whichwasdoneatthelightmicroscopiclevel,itwasshownthatthenumberofneuronsdecreasedlinearlyasrifampicindoseincreasedwhencomparedwiththecontrolgroup.CONCLUSION:Basedonthesefindings,low-doserifampicin(50μg/0.1mL)maybeusefulfortreatmentoftheoculardiseases.