简介：Thenonchromatinproteinousresidueofthecellnucleuswasrevealedinourlaboratoryasearlyasin1948andthenidentifiedbylightandelectronmicroscopyasresidualnucleoli,intranuclearnetworkandnuclearenvelopebefore1960,Thisstructuretermedafterwardsas'nuclearresidue','nuclearskeleton','nuclearcage','nuclearcarcass'etc.,wasmuchlater(in1974)isolated,studiedandentitledas'nuclearmatrix'byBerezneyandCoffey,towhomthediscoveryofthisresidualstructureisoftenwronlyascribed.Therealhistoryofnuclearmatrixmanifestationisreportedinthispaper.

简介：Thematrixmetalloproteinases(MMPs)areafamilyofzine-dependentendopeptidasesthatplayakeyroleinbothnormalandpathologicalprocessesinvolvingtissueremodelingevents.Theexpressionoftheseproteolyticenzymesishighlyregulatedbyabalancebetweenextracellularmatrix(ECM)depositionanditsdegradation,andiscontrolledbygrowthfactors,cytokines,hormones,aswellasinteractionswiththeECMmacromolecules.Furthermore,theactivityoftheMMPsisregulatedbytheirnaturalendogenousinhibitors,whicharemembersofthetissueinhibitorofmetalloproteinases(TIMP)family.Inthenormalmammarygland,MMPsareexpressedduringductaldevelopment,lobulo-alveolardevelopmentinpregnancyandinvolutionafterlactation.Underpathologicalconditions,suchastumorigenesis,thedysregulatedexpressionofMMPsplayaroleintumorinitiation,progressionandmalignantconversionaswellasfacilitatinginvasionandmetastasisofmalignantcellsthroughdegradationoftheECMandbasementmembranes.

简介：Matrixmetalloproteinases(MMPs)andtissueinhibitorsofmetalloproteinases(TIMPs)playasignificantroleinregulatingangiogenesis,theprocessofnewbloodvesselformation.Interstitialcollagenase(MMP-1),72kDagelatinaseA/typeIVcollagenase(MMP-2),and92kDAgelatinaseB/typeIVcollagenase(MMP-9)dissolveextracellularmatrix(ECM)andmayinitiateandpromoteangiogenesis.TIMP-1,TIMP-2,TIMP-3,andpossibly,TIMP-4inhibitneovascularization.Anewparadignisemergingthatmatrilysin(MMP-7),MMP-9,andmetalloelastase(MMP-12)mayblockangiogenesisbyconvertingplasminogentoangiostatin,whichisoneofthemostpotentangiogenesisantagonists.MMPsandTIMPsplayacomplexroleinregulatingangiogenesis.Anunderstandingofthebiochemicalandcellularpathwaysandmechanismsofangiogenesiswillprovideimportantinformationtoallowthecontrolofangiogenesis,e.g.thestimulationofangiogenesisforcoronarycollateralcirculationformation;whiletheinhibitionfortreatingarthritisandcancer.

简介：peritrophic矩阵(下午)为昆虫是必要的它由食物粒子，病原体，和毒素保护midgut上皮免受损坏的伤害的消化系统生理学。下午也由于它的peros可接近性是为新害虫控制策略的开发的一个吸引人的目标。理解PM怎么执行这些函数，PM的分子的体系结构用genomic被检验，proteomic在Mamestraconfigurata来临(鳞翅目：Noctuidae)，饰有十字架的oilseed的一个主要害虫在北美洲收割。PM的液体层析双人脚踏车团spectrometry分析识别了是分类的82蛋白质：(i)peritrophins，与一个CBDIII领域包括一个新班；(ii)酶在几丁质修正(几丁质deacetylases)包含了，消化(丝氨酸朊酶，aminopeptidases，carboxypeptidases，脂肪分解酵素和α-amylase)或另外的反应(β-1,3-glucanase，碱的磷酸酶，dsRNase，虾红素，pantetheinase)；(iii)没有已知的orthologs，由polycalin，REPAT，serpin，C类型lectin和Lsti99/Lsti201和3新奇蛋白质组成的一个异质的组。编码下午蛋白质的基因在midgut主要被表示。cDNAs编码几丁质synthase-2(McCHS-2)，chitinase(McCHI)，和β-N-acetylglucosaminidase(McNAG)酶，在下午几丁质新陈代谢包含了，也被识别。McCHS-2表示对显示它为在PM，的几丁质合成负责的midgut特定在midgut的唯一的几丁质的材料。相反，编码chitinolytic酶的基因在多重纸巾被表示。McCHS-2，McCHI，和McNAG在喂幼虫的midgut被表示，并且唠叨活动在PM是在场的。这个信息被用来产生鳞翅类的下午建筑学的一个更新的模型。

简介：Thenuclearmatrixattachmentregions(MARs)andthebindingnuclearmatrixproteinsinthe5'-flankingcisactingelementsofthehumanε-globingenehavebeenexamined.UsinginvitroDNA-matrixbindingassay,ithasbeenshownthatthepositivestage-specificregulatoryelement(ε-PREII,-446bp--419bp)upstreamofthisgenecouldspecificallyassociatewiththenuclearmatrixfromK562cells,indicatingthatε-PREIImaybeanerythroidspecificfacultativeMAR.IngelmobilityshiftassayandSouthwesternblottingassay,anerythroid-specificnuclearmatrixprotein(ε-NMPk)inK562cellshasbeenrevealedtobindtothispositiveregulatoryelement(ε-PREII).Furthermore,wedemonstratedthatthesilencer(-392bp--177bp)upstreamofthehumanε-globingenecouldassociatewiththenuclearmatricesfromK562,HELandRajicells.Inaddition,thenuclearmatrixproteinspreparedfromthesethreecelllinescouldalsobindtothissilencer,suggestingthatthissilencerelementmightbeaconstitutivenuclearmatrixattachmentregion(constitutiveMAR).Ourresultsdemonstratedthatthenuclearmatrixandnuclearmatrixproteinsmightplayanimportantroleintheregulationofthehumanε-globingeneexpression.

简介：Chinaisoneofthelargestmeatproducingcountriesintheworld.Withthegrowingconcernforfoodsafetymoreattentionhasbeenpaidtomeatquality.Theapplicationofconventionaltestmethodsformeatqualityislimitedbymanyfactors,andsubjectiveness,suchaslongertimetopreparesamplesandtotest.Asensormatrixwasconstructedwithseveralseparateairsensors,andtestswereconductedtodetectthefreshnessofthebeef.TheresultsshowthattheairsensorsTGS2610,TGS2600,TGS2611,TGS2620andTGS2602madebyTianjinFigaroElectronicCo,LtdcouldbeusedtodeterminethedegreeoffreshnessbutTGS2442isnotsuitable.Thisstudyprovidesafoundationfordesigningandmakinganeconomicalandpracticaldetectorforbeeffreshness.

简介：ThenuclearmatrixofdiplomonadGiardialambliawasdetectedforthefirsttimewithDGDembedmentsectioning-embedmentfreeelectronmicroscopyafteraseriesofspecificextractions.TheresultshowedthatarchaezoaGiardiaLambliaalreadypossessednuclearmatrixwithinitstwonuclei.ThefinestfibrilsofthenuclearmatrixofGiardialambliaweremeasuredtobeabout11to13nminthickness.However,thenuclearlaminaandnucleolushaveneverbeenobserved.Theseresultsseemtosuggestthatnuclearmatrixisanindispensableintranuclearstructuralcomponentevenintheprimitivenucleus.

简介：人的osteosarcomaMG-63房间被5mmol/Lhexamethylene二度乙酰胺(HMBA)导致进区别。他们的原子矩阵蛋白质(NMP)有选择地被提取并且使遭到了到二维的胶化电气泳动分析。蛋白质模式的结果被Melanie软件分析。差别的点表示了NMP被切除并且使遭到了到在有胰岛素的situ消化。采指纹的肽质量的地图被MALDI-TOF-MS分析获得，并且被吉祥物工具为NCBI数据库搜索提交。有12个点，在HMBA，其九被识别导致的区别期间显著地改变。调整蛋白质的角色在MG-63区别期间被分析。这研究建议癌症房间的导致的区别被NMP的变化伴随，并且证实与癌症房间增长和区别有关的一些特定的NMP的存在。改变的NMP为癌症治疗是为癌症诊断或目标的潜在的标记。

简介：Theeventsofcelldeathandtheexpressionofnuclearmatrixprotein(NMP)havebeeninvestigatedinapromyelocyticleukemiccelllineHL-60inducedwithetoposide.BymeansofTUNELassay,thenucleidisplayedacharacteristicmorphologychange,andtheamountofapoptoticcellsincreasedearlyandreachedmaximunabout39%aftertreatmentwithetoposidefor2h.NucleosomalDNAfragmentationwasobservedaftertreatmentfor4h.ThemorphologicalchangeofHL-60cells,thus,occurredearlierthantheappearanceofDNAladder.Totalnuclearmatrixproteinswereanalyzedby2-dimensionalgelelectrophoresis.Differentialexpressionof59nuclearmatrixproteinswasfoundin4hetoposidetreatedcells.Westernblottingwasthenperformedonthreenuclearmatrixacssociatedproteins,PML,HSC70andNuMA.TheexpressionofthesuppressorPMLproteinandheatshockproteinHSC70weresignificantlyupregulatedafteretoposidetreatment,whileNuMA,anuclearmitoticapparatusprotein,wasdownregulated.Theseresultsdemonstratethatsignificantbiochemicalalterationsinnuclearmatrixproteinstakeplaceduringtheapoptoticprocess.