简介：AbstractThis study aimed to describe a case of a patient with the spontaneous dissolution of zona pellucida (ZP) during cleavage embryo development after intracytoplasmic sperm injection (ICSI). This patient experienced early abortion due to fetal chromosome 8 abnormality and received ICSI at 31 years of age. Time-lapse monitoring of embryo cleavage and blastocyst culture was performed, and we found that the ZP of embryos 1, 2, 4, and 5 dissolved gradually during the cleavage stage of embryo development. Five blastocysts that developed from 2 pronuclear zygotes were analyzed using next-generation sequencing, and one frozen-thawed blastocyst was transferred, resulting in intrauterine pregnancy. The results of amniotic fluid puncture showed normal fetal chromosomes. To our knowledge, this is the first report of ZP dissolution during the cleavage-stage embryo; however, the exact mechanism remains unknown. The morphological changes inferred that the granular matter in the periocular space was related to the dissolution of ZP through time-lapse observation.

简介：用ＥＤＴＡ可增强Ｆｅ２＋催化鲁米诺与溶解氧的发光反应，将ＥＤＴＡ加入鲁米诺溶液中可使流动注射分析测亚铁的检出限降低约１６０倍。在流动注射分析的试样分流路中使用新型的锌镀铜微还原柱，可同时测定Ｆｅ２＋和Ｆｅ３＋。该微还原柱可至少测定３０００个试样。测定Ｆｅ２＋和Ｆｅ３＋的线性范围是１×１０９～１×１０５ｍｏｌ·Ｌ１，检出限分别为２．７×１０１０和３．５×１０１０ｍｏｌ·Ｌ１，测定试样的速率为６０ｈ１。Ｃｒ３＋和Ｃｏ２＋有干扰。测定混合物中的Ｆｅ２＋和Ｆｅ３＋获得满意结果，测试样的结果同标准的分光光度法结果一致。实验表明，ＥＤＴＡ起增强剂的作用，Ｆｅ２＋是催化剂，而溶解氧是氧化剂。对反应机理进行了讨论。