简介：Cd忍耐和米饭幼苗的translocation上的H2O2预告的处理的效果用在Cd忍耐不同的二米饭栽培变种(N07-6和N07-63)被学习。malondialdehyde(MDA)的内容，减少的谷胱甘肽(GSH)，非蛋白质thiols(NPT)，phytochelatins(PC)和谷胱甘肽S-transferase(GST)的活动在暴露于各种各样的处理的二栽培变种之间被比较。结果证明50mol/LCd暴露显著地禁止了米饭生长，提高了GSH，NPT，PC和MDA的生产，并且增加了GST的活动，并且二栽培变种之间有重要差别。更多的Cd被搬运进N07-6的射击。H2O2预告的处理由进一步在根增加GSH，NPT和PC内容，以及GST活动减轻了Cd毒性。在N07-63的这些参数的增加度比在N07-6的那些高，建议N07-63的忍耐比N07-6更显著地被提高。氢过氧化物把Cdtranslocation归结为米饭射击，但是不同地在根影响了Cd内容。从上述结果，在到在二栽培变种之间的H2O2预告的处理的Cddetoxification和反应有显著差别，这可以被推测。

简介：Introgressionlines(ILs)derivedfrominterspecificcrossesareasourceofnewgeneticvariability.Atotalof55ILsderivedfromtwocrossesSwarna×O.nivaraIRGC81848(populationA)andSwarna×O.nivaraIRGC81832(populationB)werecharacterizedforyieldandyield-relatedtraits/QTLs.Segregationof103simplesequencerepeat(SSR)markersassociatedwithyield-relatedQTLswasstudied.PopulationAshowedanaverageof12.6%homozygousO.nivaraallelesandpopulationBshowed10.6%.Interestingly,threeSSRmarkers,RM223,RM128andRM517,showedconspicuouspatternofsegregation.ThedistributionofparentalallelesatthreelociRM223,RM128andRM517linkedtoyield-relatedtraitswasunique.Thesemarkersflankedtoseveralyield-relatedQTLs.RM223,flankingtoqyld8.3,washeterozygousinalmostallthe55ILsexceptinIL10-3SandIL131S.RM128onchromosome1andRM517onchromosome3weremutuallyexclusivein46outof55ILs.These46ILsshowedeitherofthemarkeralleleRM128orRM517fromO.nivarabutnotboth.IL166ShadbothRM128andRM517fromO.nivaraandtheotherILsshowedhomozygousSwarnaalleleatRM517exceptIL65S.Populationstructureassignedthe55ILstothreesub-populationsbasedontheirgenomicdiversity.IL65S,IL166S,IL248S,IL7KandIL250Kshowedhighyieldsinmulti-locationtrials,andIL248SwasreleasedforcultivationasDRRDhan40.

简介：为谷物矿物质元素的量的特点loci(QTL)的鉴定能通过帮助标记的繁殖在微量元素的更快、更精确的开发帮助稠密的米饭变化。在现在的学习，QTL在二BC2印射人口的F3源于O的十字。有O的二不同就职的sativacvSwarna。nivara。总共，10和8QTL为谷物Fe被识别，在人口1，和7和5QTL的Zn集中分别地在人口2被识别。在两张人口检测的百分之八十QTL从O被导出。nivara。为Fe的五QTL和为Zn的三QTL也在印射的间隔或合成间隔向超过15%phenotypic解释了变化。O的地点。nivara导出象qFe2.1，qFe3.1，qFe8.2和qZn12.1那样的QTL一致地在两个被识别人口。Epistatic相互作用在人口1在为Fe集中的染色体3上在染色体2上并且在RM22和RM7之间仅仅在RM106和RM6之间被观察。为金属动态平衡的十六候选人基因被发现在两个为Fe和Zn集中与10QTL驻扎在同一地点人口。大多数Fe和ZnQTL被发现为谷物产量和谷物质量特点与QTL驻扎在同一地点。一些识别的主要效果QTL能被用来改进米饭谷物Fe和Zn集中。

简介：阳离子exchangers(CAX)属于广泛地在最后十年在植物tonoplasts被调查了的cation/Ca2+exchanger总科。最近，在植物涉及重金属累积和忍耐的CAX的角色为植物救治和食物安全被学习了。在这微型评论，我们在Ca2+信号transduction总结Ca2+/H+antiporter的角色，维持离子动态平衡并且扣押重金属进液泡。而且，我们在场在重金属detoxification的血浆膜Ca2+/H+antiporter的一个可能的角色。

简介：Twostarch-branchingenzyme(SBE)inrice,isknowntobeakeyenzymeinamylopectinbiosynthesis.ThecDNAoftwoSBE(starch-branchingenzyme)genesSheIandShedencodingSBEⅠandSBEⅢ(twomajorisoformsinrice)wereclonedbyanimprovedRT-PCRtechnique,fromatemplatecDNAlibray,derivedfromthetotalmRNAsextractedfromtheimmatureseedsofajaponicariceWuyunjing7.DNAsequenceanalysisshowedthatthesizeoftheclonedSheIandShedcDNAswere2490and2481bplong,respectively,includingtheirentirecodingsequences.ComparisonanalysisindicatedthatthenucleotidesequenceofShe3wasthesameasthatofshed(GenbankAccessionNo.D16201)asreportedpreviously.Therewereonlyfourbase-pairsdifference,whichresultedinchangesoftwodeducedaminoacidsbetweentheclonedShe1cDNAandthereportedshe1(GenbankAccessionNo.D11082).TheclonedSheIandShedcDNAsmakeitpossibletoimprovericestarchqualitythroughgeneticengineering.

简介：Lipoxygenase3(LOX3)isamajorcomponentoftheLOXisozymesinmaturericeseeds.ToinvestigatetheroleofLOX3geneunderstresses,aplantexpressionvectorcontainingantisensecDNAofLOX3wasconstructed.RicevarietiesWuyunjing7andKasalathweretransformedbytheAgrobacterium-mediatedmethodandtransgenicriceplantsweregenerated.PCRandSouthernblotresultsshowedthattheantisenseLOX3genewasintegratedintothericegenome.AnalysesofembryoLOX3deletionandsemi-quantitativeRT-PCRconfirmedtheantisensesuppressionofLOX3geneintransgenicplants.TheT2antisenseplantsofLOX3weresensitivetodroughtstress,riceblastandbacterialblightcomparedwithnon-transgenicplants.TheseresultssuggestthattheLOX3genemightfunctioninresponsetostresses.

简介：TounderstandthewildOryzagenomeeffectonphotosynthesisanditsrelationtototaldrymatteraccumulationinanelitericevariety,asetof40stableintrogressionlines(ILs)BC3F8derivedfromacrossofOryzasativa(KMR3)×Oryzarufipogon(WR120)weregrownunderwellwateredconditions.Leafgasexchangemeasurementsandleafchlorophyllestimateswereconductedatthefloweringstage.Theresultsrevealedsignificantvariationsinnetphotosyntheticrate(Pn),transpirationrate(E),transpirationefficiency(Pn/E)andcarboxylationefficiency(Pn/Ci).PnshowedsignificantpositivecorrelationwithE,stomatalconductance(gs),Pn/Ciandtotalcanopydrymatter.SpecificleafareaandleafthicknesswerenotsignificantlycorrelatedwithPn.Thirty-sevenoutof40ILsshowedhigherPnthanKMR3[11.28μmol/(m2·s)],and20ILsshowedhigherPnthanWR120[15.08μmol/(m2·s)].ThelineIL194showedthehighestPn[21.62μmol/(m2·s)]withincreasedtotalcanopydrymatterfollowedbylinesIL381,IL106,IL363-12,IL198,IL86-18andIL50,whichexhibitedPnabove18.0μmol/(m2·s).TheILswithenhancedPnareapotentialsourcefordevelopingricevarietiesandhybridswithhigherbiomassandyield.

简介：TheinteractionbetweenricehostanditspathogenXanthomonasoryzaepv.oryzae(Xoo)atcellularlevelwasstudiedbyusingaresistantsomaclonalmutantHX-3anditssusceptabledonorMinghui63.AfterinoculationwithXoostrainZhe173(Chinesepathotype|\).theactivityofsuperoxidedismutase(SOD)andperoxidase(POD)inthecallusofMinghui63wasincreaseddramatically,andtheactiveoxygen(O2^-)wasproducedatahigherrate;Meanwhile,thecallusgrewslowlywiththereductionofproteincontent.ComparedtotheactivityofSODandPOD.theproductionrateofO2^-andthefreshweightinHX-3callusvariedlittleaftertheinoculation.ItcouldbeproposedthatthereweregreatdifferencesbetweentheresistanceofHX-3andMighui63atcellularlevel.TherewasnodifferencedetectedconcerningresistancetobacterialleafblightinHX-3betweentheplantandthecallus.

简介：到精选精英germplasms，从装饰用的梨树米饭栽培变种Wuyujing3导出的112异种被评估。象圆锥花序那样的收益部件每平方米数，谷物数字每圆锥花序，和谷物，重量被测量。象白垩的谷物(PCG)的百分比那样的优秀特点，稻谷收益(BRY)，milled米饭收益(MRY)，milling(DM)的度，直链淀粉内容(交流)，蛋白质内容(PC)，和在特点之中的关系是inverstigated。结果证明谷物产量每在谷物收益变化为94.64%贡献的平方米与6.4t/hm2的一个平均数和谷物的数字从2.15～12.49t/hm2。为优秀特点，所有米饭异种有短尺寸(谷物长度≤5.5 ；公里)并且大胆形状(到宽度比率=1.10-2.00的谷物长度)。大多数米饭异种(87.5%)低于20%有PCG价值。有的MRY重视甚于50%的所有异种，低于20%的交流值，和低于10%的PC值。白垩的谷物的百分比否定地显著地与MRY被相关并且断然与DM相关。BRY和MRY否定地显著地与DM被相关。PC显著地并且断然与MRY被相关并且否定地与DM相关，当交流没这些优秀特点地有重要关联时。有25米饭异种，完成了象白垩的谷物的低百分比那样的江苏标准装饰用的梨树米饭的主要要求，这被结束，低直链淀粉满意的、最佳的蛋白质内容，并且它能被用作精英germplasms。因此，识别的异种可以在米饭质量的改进导致重要进步。

简介：为了推进，改进高地大米变化Huhan3和Huhan7，种子样品为约1和5d与二艘可重获的飞船被送到外层空间并且分别地为7和5代被宣传。Phenotypic分析表明词法特点和蛋白质和谷物的直链淀粉内容变化了。由联系基因的简单顺序重复(SSR)和插入删除(InDel)的genomic变化的描述标记显示变化模式是很复杂的。大多数变化在简单顺序重复碎片发生在碎片的3结束或5结束。反向的抄写聚合酶链反应(RT-PCR)试金证明在SSR的那些部分的变化影响了他们的基因表示，显示那基因联系了标记将有用孤立功能的基因。为繁殖的地调查也表明有高产量，高质量和干旱忍耐的更多的线能通过太空繁殖被选择。结果显示太空mutagenesis为米饭繁殖导致了分子的变化，以及生理、词法的变化。

简介：一张加倍的haploid(DH)人口由120根线组成了，源于在一个indica变化，TN1，和一个装饰用的梨树变化之间的一个十字，Chunjiang06，被用来识别由使用QTLNetwork软件在胚芽和3-leaf-seedling阶段控制米饭寒冷忍耐的QTL。在4以后的正常胚芽的百分比

简介：到怀有基因Pi-d2从pCB6.3kb，pCB5.3kb和pZH01-2.72kb的三不同表示向量转变了的米饭强风抵抗的转基因的米饭线的米饭强风的抵抗被分析。有Pi-d2基因的九根先进产生的转基因的米饭线显示了各种各样的抵抗到39米饭强风紧张，并且最高疾病抵抗的频率到达了91.7%。有Pi-d2基因的四根早产生的同型结合的转基因的线展出了抵抗到58米饭强风紧张中的超过81.5%个，显示出宽光谱的抵抗的特征。当在文化媒介的粗略的毒素的集中增加了，米饭强风真菌的粗略的毒素选择的转基因的胚胎的calli证明从转基因的米饭植物的不成熟的胚胎的胼胝正式就职率减少了。当粗略的毒素的集中到达了40%时，从转基因的线的不成熟的胚胎的胼胝正式就职率是49.3%，并且受体控制的是5%。在正式就职下面的地里的转基因的大米线的颈强风的疾病发生是0%～50%，显示转基因的大米线的大米强风抵抗比受体控制的高得多。

简介：Tiller角度，一个很必要的农学的特点，在米饭繁殖是重要的，特别在植物类型繁殖。控制2的一个tiller角度(tac2)异种被乙醇甲烷磺酸盐mutagenesis从restorer线Jinhui10获得。tac2异种在幼苗阶段和显著地在tillering阶段增加的tiller角度显示了正常显型。初步的生理的研究显示异种对GA敏感。因此，TAC2和TAC1可能以一样的方法控制tiller角度，这被推测。基因分析证明变异的特点被主要后退的基因控制并且位于用SSR标记的染色体9。在TAC2和它的最近的标记RM3320和RM201之间的基因距离分别地是19.2厘米和16.7厘米。

简介：Byusing304recombinantinbredlinesderivedfromindicaricecrossZhong156/Gumei2,alinkagemapconsistingof177markerlociandcovering12ricechromosomeswasconstructedandemployedformappinggenesconferringblastresistanceinrice.GenomiclocationofgenePi25(t)conferringneckblastresistancetotheChineseisolate92-183(raceZC15)wasverifiedtobelocatedbetweenmarkersA7andRG456onchromosome6,withgeneticdistancesof1.7cMand1.5cMtoA7andRG456,respectively.LeafblastresistanceofGumei2tothePhilippineisolateCa89(lineage4)wasfoundtobecontrolledbyasinglegene.ThegenetentativelydesignatedasPi26(t)waslocatedbetweenmakersB10andR674onchromosome6,withgeneticdistancesof5.7cMand25.8cMtoB10andR674respectively.ResistantallelesatbothgenelociwerederivedfromGumei2,indicatinganexistenceofresistancegeneclusterinGumei2.

简介：到在米饭germplasm91-1A2的米饭胆量小蚊的抵抗被识别并且遗传上分析了。米饭人口的F1s从作为一个男父母与米饭材料Jinggui，TN1，W1263(Gm1)，IET2911(Gm2)，BG404-1(gm3)，OB677(Gm4)，ARC5984(Gm5)和Duokang1(Gm6)交叉的91-1A2被导出。到米饭胆量小蚊的所有父母线和F1，BC1F1和F2人口的抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1和F2的易受影响的抵抗植物的分离比率被X2测试与1:3和9:7规则给予，建议到中国米饭胆量小蚊遗传因子型IV的91-1A2的抵抗被是新抵抗基因的二主导的基因控制，对已知的米饭胆量小蚊抵抗基因非突变而产生之遗传因子。

简介：Thetransgenicrice,Zhongda2,whichwasgeneticallymodifiedfromanindicaricelineZhuxianBbyricechitinasegene(RC24),hadhighresistancetoricesheathblight(Rhizoctoniasolam)inlaboratoryandatwo-yearfieldexperiment.ThepathogencouldinvadesheathofZhongda2andinducesymptomsofthedisease.NodifferencewasnotedintimeofpenetrationorincubationperiodbetweenZhongda2andnon-transgenicricecontrol,ZhuxianB,butthehyphaelysatecouldbeobservedeadierthancontrol.Itsresistanceexpressedastoinhibitthegrowthofmyceliuminhosttissue.F1sfromZhongda2(♂)crossedwithotherfivenon-transgenicricelinesshowedhigherresistancethandonornon-transgenicparents,buttheresistancewasdifferentalongwiththedifferentmaternalparents.

简介：Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.

简介：Bacterialleafblightofrice(BLB),causedbyXanthomonasoryzaepv.oryzae,isoneofthemostdestructivediseasesinAsianricefields.Ahigh-qualityricevariety,LT2,wasusedastherecipientparent.IRBB21,whichcarriestheXa21gene,wasusedasthedonorparent.TheresistancegeneXa21wasintroducedintoLT2bymarker-assistedbackcrossing.ThreeXooraceswereusedtoinoculatetheimprovedlinesfollowingtheclippingmethod.ElevenBC3F3linescarryingXa21wereobtainedbasedonmolecularmarkersandagronomicperformance.The11linesweretheninoculatedwiththethreeXooraces.Allthe11improvedlinesshowedbetterresistancetoBLBthantherecipientparentLT2.BasedonthelevelofresistancetoBLBandtheiragronomicperformance,fivelines(BC3F35.1.5.1,BC3F35.1.5.12,BC3F38.5.6.44,BC3F39.5.4.1andBC3F39.5.4.23)wereselectedasthemostpromisingforcommercialrelease.Theseimprovedlinescouldcontributetoriceproductionintermsoffoodsecurity.

简介：布朗planthopper(Nilaparvatalugens圣?l)大多数损坏害虫之一在米饭正在引起hopper灼伤，并且从而减少生产率并且另外产品的质量。控制这个害虫的有效管理策略是到本地米饭栽培变种的理想的基因的鉴定和转移。为开发抵抗栽培变种的最重要的途径是标记的鉴定，它能在更持久的抵抗遗传型的帮助标记的选择帮助。易受影响的父母IR50和抵抗父母Ptb33，和他们的F2人口是为有随机的放大多态的DNA的抵抗基因的鉴定的使用的inbulkedsegregant分析标记(RAPD)教材。教材OPC7和OPAG14证明主导、易受影响的特定的banding模式那么叫了co主导的标记。而且，OPC7697和OPAG14680给抵抗特定的乐队看了并且因此在联合分阶段执行，而OPC7846和OPAG14650给易受影响的特定的genotypic乐队看了inbulkedsegregant分析。因此，联合阶段标记，OPC7697和OPAG14680，被认为在在庄稼改进的米饭遗传型的帮助标记的选择更有用。