简介：Objective:Toobservetheeffectivenessofhighlyactiveantiretrovirustherapy(HAART)onHIV/AIDSpatients.Methods:UsingHIV-Iquantitativemethodsandimmunologicalfunctioninspection,wemonitored4HIV/AIDSpatientswhoweresufferingfromimmunologicaldeficiencyandweretreatedwithHAART.Results:ThereproductionofHIVinall4patientswasefficientlycontrolledatthe4thweekofthetreatment.Theaverageviralloaddecreasedby1.99Log/ml(0.73-2.46Log/ml).ThenumberofCO+4andCD+sshowedasteadycontinuousincrease4to12weeksafterthetreatment,withanincreaseof67.2%and103.0%respectively.CorrelativestudyamongdifferentvariablesafterthetreatmentrevealedthatpositivecorrelationexistedbetweenthenumberofCD+4andCD+3aswellasCD+8,whilenegativecorrelationexistedbetweenthenumberofCD+4andplasmaviralload.Conclusion:HIV-Iquantitativemethod(plasmaviralload)andthenumberofCD+4inperipheralbloodcanbeusedasimportantreferenceindicatorsinevaluatingHAART.

简介：Objective:Todevelopasensitive,specificandsimplemethodfordetectionofextremelylownumbersofT.palliduminclinicalspecimens,asasignificantadditiontotheserologictestsforsyphilisdiagnosis.Methods:Double-tubenestedPCR(DN-PCR)andsingle-tubenestedPCR(SN-PCR)assayswereperformedtoamplifyspecificfragmentsoftheDNApolymeraseIgene(polA)ofT.pallidum.SensitivityandspecificityofthetwoPCRassaysweretested.EightysixwholebloodspecimensfrompersonswithsuspectedsyphilisweredetectedbythetwonestedPCRmethods.TheTPPAtestwasusedasacomparisonfordetectingsyphilisinserafromcorrespondingpatients.Results:OnlyspecificampliconscouldbeobtainedduringamplificationoftheT.pallidumpolAgeneandthedetectionlimitwasapproximately1organismwhenanalyzedongelbythetwoPCRmethods.Of86clinicalspecimens,62werepositivebyTPPA.Ofthese,54and51werepositivebytheDN-PCRandSN-PCR,respectively,whichdoesnotrepresentastatisticallysignificantdifferencebetweenthetwoPCRtests.Of24TPPA-negativespecimens,5werepositivebybothDN-PCRassayandSN-PCRassay.Conclusion:TheSN-polAPCRmethodisextremelysensitive,specificandeasytoperformfordetectinglownumbersofT.palliduminclinicalbloodspecimensasacomplementarytoserologyforsyphilisdiagnosis.