简介：BACKGROUND:Highincidenceofstrokeatinterchangeperiodofautumnandwinterwasdemonstratedbyepidemiologicalsurvey,andthespecificcausesshouldbefurtherinvestigated.OBJECTIVE:Toinvestigatetheinfluenceofartificialcoldexposureontheincidenceofstrokeinrenovascularhypertensiverats(RHR),andanalyzetheassociationwithbloodpressureandcold-inducibleRNAbindingprotein(CIRP)mRNAexpressioninbraintissue.DESIGN:Acompletelyrandomizedgroupingdesign,arandomizedcontrolanimaltrial.SETTINGS:LabofNeurology,theFirstAffiliatedHospitalofSunYat-senUniversity;DepartmentofChemistry,OpenlaboratoryofChemicalBiology,InstituteofMolecularTechnologyforDrugDiscoveryandSynthesis,UniversityofHongKong.MATERIALS:MaleSDrats(n=460),weighing80-100gwereobtainedfromGuangdongProvinceHealthAnimalUnit.AmodifiedRXZ-300Aintelligentartificialclimatecabinet(NingboJiangnanInstrumentCo.,Ltd.,China).METHODS:TheexperimentwereprocessedintheLabofNeurology,theFirstAffiliatedHospitalofSunYat-senUniversityandtheOpenLaboratoryofChemicalBiology,InstituteofMolecularTechnologyforDrugDiscoveryandSynthesis,UniversityofHongKongfromOctober2004toNovember2005.Rats(n=400)wereoperatedtoestablish2-kidney2-clipRHRmodelasdescribedpreviously.Thesham-operatedrats(n=60)servedasnormotensivecontrols.Eightweekslater,300ofRHRwererandomlyselectedaccordingtotheirsystolicbloodpressure(SBP)anddividedinto3sub-groups(n=100pergroup):mildhypertensivegroup(SBPof160-200mmHg),moderatehypertensivegroup(SBPof200-220mmHg)andseverehypertensivegroup(SBP>220mmHg).Eachgroupwasfurtherdividedintotwogroups(n=50)underACEandnon-ACE.Normalsham-operatedSDrats(n=60),SBP<140mmHg,wererandomlydividedintotwogroups:Sham-operatedcontrolgroup(n=30)underACEandnon-ACE.ToestablishtheACEandnon-ACEtreatment,ratswerehousedindividuallyinartifici