简介：Osteocytesactasmechanosensorsinbone,whichcansendmechanicalsignalsdirectlytoosteoblaststhroughgapjunctions.However,underphysiologicalconditionsthenumberofgapjunctionsislimitedbecauseofthequantityvarianceofthetwokindsofcells.Inthisstudy,thepossibilityofindirectinteractionbetweenthesetwocellswasinvestigated.Anewflowchamperwasdesignedinwhichosteocytesandosteoblastswerecoculturedintwochampersseparately.OsteocyteswereexposedtofluidflowandthentheALPactivity,osteocalcinandosteopotinofosteoblastsweredetermined.TheresultsshowedthateitherALPactivityorproductionofosteocalcinandosteopotininosteoblaststhatwerecoculturedwithshearedosteocytesincreased,whichindicatedthatosteocytescouldregulateosteoblastsindirectlythroughsomesolublefactors.