简介：Si-Miao-Wan(SMW),atradiationalChinesemedicinalformulaconsistingofAtractylodisRhizoma,PhellodendriChinensisCortex,CoicisSemen,andAchyranthisBidentataeRadix,hasbeenusedforthetreatmentofgoutandgoutyarthritisformanyyears.Inthepresentstudy,aliquidchromatographyquadrupole-time-of-flightmassspectrometry(LC-Q-TOF/MS)methodwasestablishedtoidentifythemultipleconstituentsofSMWanditsmetabolitesinratbiologicalsamplesafteroraladministration.Atotalof48compoundsinSMW,including21alkaloids,12organicacids,2terpenes,3lactones,2phytosterols,and8othercompounds,weretentativelycharacterizedwiththediagnostic-ionfilteringstrategy.BasedonthediagnosticionsappliedtoidentifycompoundsinSMW,28prototypecompoundsand10metaboliccompoundsweredetectedinthebiologicalsamples.ThiswasthefirstcomprehensivedrugmetabolisminvestigationofSMWinrats.Thedevelopedmethodcouldbeausefulmeansforidentifyingthemulti-componentsinSMWandthemetaboliccomponents.TheresultsmayhelpexplorethepossiblemetabolicprocessesandmechanismofactionforSMWinvivo.

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简介：TocharacterizeandidentifymultipleconstituentsinDanhonginjection(DHI),afastultra-highperformanceliquidchromatographycoupledtoelectrosprayionizationquadrupoletime-of-flighttandemmassspectrometry(UHPLC-ESI-QTOF/MS)methodwasestablishedandvalidatedinthepresentstudy.Atotalof63compounds,including33phenolicacids,2C-glycosylquinochalcones,6flavonoidO-glycosides,4iridoidglycosides,6organicacids,5aminoacids,and3nucleosides,wereidentifiedortentativelycharacterized.Inconclusion,theUHPLC-ESI-QTOF/MSmethodisusefulandefficientforin-depthstructuralelucidationofchemicalcompoundsincomplexmatricesofherbalmedicinessuchasDHI.

简介：Thepresentstudywasdesignedtodevelopandvalidateasensitiveandreliableultrahighperformanceliquidchromatographycoupledwithquadrupoletime-of-flightmassspectrometry(UPLC-QTOF/MS)methodtoseparateandidentifythechemicalconstituentsofQixueShuangbuTincture(QXSBT),aclassictraditionalChinesemedicine(TCM)prescription.UndertheoptimizedUPLCandQTOF/MSconditions,56componentsinQXSBT,includingchalcones,triterpenoids,protopanaxatriol,flavonesandflavanoneswereidentifiedandtentativelycharacterizedwithinarunningtimeof42min.Thecomponentswereidentifiedbycomparingtheretentiontimes,accuratemass,andmassspectrometricfragmentationcharacteristicions,andmatchingempiricalmolecularformulawiththatofthepublishedcompounds.Inconclusion,theestablishedUPLC-QTOF/MSmethodwasreliableforarapididentificationofcomplicatedcomponentsintheTCMprescriptions.