简介：通讯因素分析(CFA)被采用学习14个HPLC系统的选择。测试LC系统作为反向阶段被分类(RP)，离子交换(IE)和吸水的相互作用层析(HILIC)模式。HILIC列上的吸水的溶质的保留被秒顺序效果显著地除他们的吸水的性质以外影响，这被发现。硅石表面上的器官的修饰词和残余silanol组两个都参予了保留。Hypersil？氨基的列以适当条件在HILIC模式执行了分离，并且它的保留机理比(aspartamide)HILIC，柱涂渍poly组织更类似于HILIC硅石列的。

简介：Rheumatoidfactors(RFs)arethecharacteristicautoantibodiesofrheumatoidarthritis.Recentresearchesinourlaboratoryshowedthattheimmobilizedsingle-strandedDNA(ss-DNA)immunoadsorbentcanselectivelyremoveRFsfromtheserumofpatients.Inthepresentpaperarestudiedthemodificationofargininine,tryptophan,lysineresiduesandcarboxylterminusofIgGRF,whichwasseparatedfrompatients′serum,with1,2-cyclohexanedione(CHD),N-bromosuccinimide(NBS),pyridoxal5′-phosphate(PP)and1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC)respectively,andtheireffectsontheadsorptioncapacityoftheimmobilizedss-DNAimmunoadsorbentforIgGRF.Afterthespecificmodification,thecorrespondingadsorptioncapacitiesoftheadsorbentswerechangedfrom48%,46%,44%and54%to84%,14%,21%and81%,respectively.Theseresultsindicatethattheelectrostaticorionic-bondingisessentialfortheinteractionbetweenss-DNAandIgGRF.

简介：OrganicmodifierswereeffectivebothtoextendthemigrationtimewindowandtoimprovetheseparationofveryhydrophobiccompoundsinMEKC.Aniterationmethodwasusedtodeterminethemigrationtimeofmicelles.Thequantitativerelationshipbetweenthecapacityfactork’andtheconcentrationoforganicmodifierswasderived,whichwasinvestigatedexperimentally.Thelinearsolvationenergyrelationships(LSER)methodologywasappliedtoMEKC,andgoodlinearrelationshipsbetweenInk’andsolvatochromicparametersof15soluteswereobtainedinthepresenceoforganicmodifierindifferentconcentrations,whichindicatedanewaccessinMEKCtopredictk’fromthestructuralparametersofsolutes.TheeffectofcolumntemperatureTonk’wasalsoinvestigated.