简介:GracilariatenuistipitatavarliuiZhangetXiaiscultivatedattwodifferentsalinities(21,33)inthelaboratoryfor4weeks.Thedailygrowthrateisdetermined.Thetotalagaryieldandfractionalagarcompositionareanalyzed.Resultsshowthatalgaegrowfasterinlowsalinity.Thetotalagaryieldishigherunderhighsalinityconditionsthanunderlowsalinityconditions.Amongtheeightfractionsextracted,theyieldsofcoldwaterextractand40%ethanolextractareaboutthehighest.Inlowsalinitiestheyieldsofautoclavedextractand60%ethanolextractarehigher,whiletheyieldofcoldwaterextractislowerrelativetohighsalinities.
简介:Appropriatepathogenicbacteriawereisolatedfromdiseased(ordead)flounder(ParalichthysolivaceusL)occurringinamariculturefarmsofQinhuangdao,whichalldiseasedfishesexpressedbacterialseptieaemia.Thephenotypicinformationofthe12purecultureswasstudiedextensively,includingmorphologicalcharacteristics,colonycharacteristicsindifferentmedia,physiologicalandbiochemicalcharacteristics,andthemolefractionG+CratiooftheDNAforrepresentativestrain.TheresultsshowthattheisolatesbelongtoanewspeciesofVibrio,andaredesignatedasVibrioqinhuangdaorasp.nov.bytheareafromwhichthediseasedfloundersarecollected(Qinhuangdao)basedonitsbiologicalpropertiesfollowingRulesofInternationalCodeofNomenclatureofBacteria.Atthesametime,studiesontheserumhomologyofisolates,andpathogenicityofisolateswereconductedbyexperimentalinfection.Theresultsshowthatalltheisolatesareofserologicsimilarity,andtheisolateshavestrongpathogenicitytoflounder.ThestudiescanextensivelyreflectthemainbiologicalpropertiesofthisnewspeciesofVibrio.