简介：ObjectiveTostudytheeffectofsalicylateontheexpressionandfunctionofNMDAreceptorsinspiralganglionneurons(SGNs).MethodsThemRNAofNR1subunitofNMDAreceptorinmodiolustissuesweredetectedbyRealtimefluorescencequantitativePCR(FQ-PCR).NMDAreceptorwhole-cellcurrentswererecordedusingpatchclampinacuteisolatedSGNs.ResultsComparedwiththecontrolgroup,salicylatesignificantlyincreasedthemRNAlevelofNR1subunitinSGNs.NMDAofconcentrationsrangingfrom0.1mMto10mMevokednocurrentinSGNs.NMDA(0.1mMand0.5mM)appliedwithsalicylate(5mM),however,inducedinwardcurrents(212.6±15.2pA,n=5;607.9±44.3pA,n=5)inadose-dependentmanner,whichcouldbeinhibitedbyAPV.SalicylatealonedidnotproduceanycurrentinSGNs.ConclusionSalicylateincreasestheexpressionofNMDAreceptorsandfacilitatesthecurrentsmediatedbyNMDAreceptorsinSGNs.