简介:摘要目的分析Na+/H+交换蛋白1(NHE1)抑制剂对癌基因BRAF野生型(BRAFWT)和激活型BRAFV600E突变的胶质母细胞瘤(GBM)细胞生长和侵袭能力的影响。方法NHE1抑制剂Cariporide分别处理U251(BRAFWT)和AM38(BRAFV600E)GBM细胞系,乙酰甲酯化的2’,7’-双(2-羧乙基)-5(6)-羧荧光素荧光探针处理细胞并采用紫外分光光度计检测细胞在440 nm与490 nm的荧光强度,计算荧光强度比值以反映NHE1的活性,MTT法检测细胞增殖活性,基质胶-Transwell实验检测细胞侵袭能力。结果AM38细胞的NHE1活性、增殖和侵袭能力均显著高于U251细胞,差异均有统计学意义(P=0.006、0.010、0.047);Cariporide处理的U251和AM38细胞的NHE1活性、增殖和侵袭能力均显著低于溶剂二甲基亚砜处理的U251和AM38细胞,差异均有统计学意义(U251:P=0.012、0.023、0.044;AM38:P=0.006、0.001、0.038)。结论采用Cariporide阻断NHE1活性可有效抑制BRAFWT和BRAFV600E突变型GBM细胞的增殖和侵袭。
简介: 【摘要】 目的:探讨糖尿病肾病血液透析患者应用预见性护理的效果。方法:选取 2017年 1月 -2019年 1月笔者所在医院 114例糖尿病肾病患者,所有患者均采用血液透析治疗,将其随机分为观察组与对照组,各 57例。对照组给予常规护理,观察组应用预见性护理。比较两组护理效果。结果:观察组低血糖及院内感染发生率分别为 3.51%、 7.02%,均明显低于对照组的 15.79%、 31.58%,差异有统计学意义( P<0.05)。护理后,观察组空腹血糖、餐后 2 h血糖及糖化血红蛋白水平均明显低于对照组,差异有统计学意义( P<0.05)。结论:对糖尿病肾病血液透析患者实施预见性护理,对低血糖与院内感染的发生有积极预防作用,且可提高对患者血糖水平的控制效果,值得推广。 【关键词】 糖尿病肾病 血液透析 预见性护理 低血糖 院内感染 [Abstract] Objective: To explore the effect of predictive nursing in hemodialysis patients with diabetic nephropathy. Methods: from January 2017 to January 2019, 114 patients with diabetic nephropathy in our hospital were randomly divided into observation group and control group, each with 57 cases. The control group was given routine nursing and the observation group was given predictive nursing. The nursing effect of the two groups was compared. Results: the incidence of hypoglycemia and nosocomial infection in the observation group were 3.51% and 7.02%, respectively, which were significantly lower than that in the control group (15.79% and 31.58%) (P < 0.05). After nursing, the levels of fasting blood glucose, postprandial blood glucose and glycosylated hemoglobin in the observation group were significantly lower than those in the control group (P < 0.05). Conclusion: predictive nursing for hemodialysis patients with diabetic nephropathy can prevent the occurrence of hypoglycemia and nosocomial infection, and improve the control effect of blood glucose level, which is worth popularizing.