An Experimental Study on the Flexibility of Prevention against Thrombosis Following Mechanical Valve Replacement by tPA Gene Transduction-中国期刊网

摘要 Useagenesutureimmersedrecombinanttissue-typeplasminogenactivator(r-tPA)expressionplasmidtotransducemyocardiatopreventthethrombosisaftermechanicaltricuspidvalvereplacementinpigs.MethodsAr-tPAgeneplasmidwasconstructedandconjugatedtoanovelcationicphosphonolipidandar-tPAgenesuturewasmade.Eighteenpigswereselectedanddividedintotwogroupsatrandomization.Therewere9pigsintheexperimentalgroupand9inthecontrolgroup,allthe18pigs'tricuspidswerereplacedwithmechanicalvalves.Thegenethreadsweresuturedintotherightventricularwallsnearmechanicalvalvesandanultrasoundwasusedonthesurfacesoftherightventricularwallsforthegenetransferintheexperimentalgroup.Coagulativefunction,D-dimerlevelofthebloodandthethrombosisonthesurfacesofthevalveswereobserved.Resultsr-tPAgeneplasmidwassuccessfullyconstructedandr-tPAproteinwasexpressedintheventricularcellsaroundthegenesutures.D-dimerreacheditspeaklevel(1.67±0.79)μg·mL-1in1weekafteroperationintwogroups,butitdecreasedtopreoperationlevelthereafterincontrolgroupandkeptonthehighlevelandreincreasedtoanewhighlevel(1.89±0.79)μg·mL-1untiltheendoftheexperimentinexperimentalgroup.Thethrombosesaroundthevalveswerefoundinallthecontrolgroup(100%)butonly1(11.11%)caseinexperimentalgroup.Therewerenochangesinprothrombintimepreandpostoperationintwogroups.ConclusionsUsinggenesutureimmersedr-tPAexpressionplasmidtotransducemyocardiamightbeabestsubstitutionforlifelonganti-coagulationtherapyforthepatients,whounderwentoperation.

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An Experimental Study on the Flexibility of Prevention against Thrombosis Following Mechanical Valve Replacement by tPA Gene Transduction

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An Experimental Study on the Flexibility of Prevention against Thrombosis Following Mechanical Valve Replacement by tPA Gene Transduction

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