Isolated Cells of Porphyra yezoensis Cultured on Solid Medium-中国期刊网

摘要 VegetativecellsofPorphyrayezoensisareisolatedwithseasnailenzymeandculturedonthesolidifiedagarmedium.Theresultsofexperimentsshowthattheisolatedcellscansurvive,divideandregeneratewellonthemediumsolidifiedwithagar.Thefirstdivisiononthesolidmediumstartsafter7days'culture,4dayslaterthantheliquidculture.Thesurvivalrateofisolatedcellsis71.3%onthesolidmedium,lowerthanthe86.2%ofthatinseawater.Thalli,thalloids,conchocelis,spermatangiaandmulticellularmassesaredevelopedonthesolid/mediuminthefirstmonth,slowlybutnormally.Spermatangiasacsdisappearwithin4weeks.Withoutaddingnutrientliquidontothesurfaceofsolidmediumorinjectingseawaterundertheagarlayerinordertokeepmoisture,thethalliandcellgroupsreleasemonosporestoformnewthalliinsteadofenlargingtheirareasafter5weeks'culturing.Somemonosporesregeneratenewthalli.Othermonosporeslosetheirpigmentsandminimizetheirvolumeanddividequicklytoformlightpinkcalli.After16weeks,numerouscallicanbeseenonthesolidmediumandafter24weeks'culturing,almostonlycalliandconchoceliscanbeseen.Ifthecalliareimmersedinseawater,themonosporesarereleasedandmaydevelopintoyoungthallus.

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Isolated Cells of Porphyra yezoensis Cultured on Solid Medium

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Isolated Cells of Porphyra yezoensis Cultured on Solid Medium

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