摘要
TheaimofthisstudyistoexplorepotentialpathogenicityofMycoplasmapenetrans,andtoinvestigatewhetherM.penetranslipid-associatedmembraneproteins(LAMPs)couldinducehumanmonocyticcellline(THP-1)toproducesomeproinflammatorycytokinesinvitro,includinginterleukin-1β(IL-1β),tumornecrosisfactoralpha(TNF-α),andIL-8.THP-1wasstimulatedwithdifferentconcentrationsofM.penetransLAMPsandatdifferenttimetoanalyzetheproductionofhumanIL-1β,TNF-αandIL-8.TheproteinlevelsofhumanIL-1β,TNF-αandIL-8weremeasuredbyenzyme-linkedimmunoadsorbentassay(ELISA)andthemRNAlevelsoftheseproinflamrnatorycytokinesweredetectedbyreversetranscriptase-PCR(RT-PCR).ItwasdemonstratedinthepresentstudythattheproductionofIL-1β,TNF-αandIL-8increasedindose-andtime-dependentmannerafterstimulationwithM.penetransLAMPsinTHP-1cells.M.penetransLAMPsalsoinducedtheexpressionofIL-1β,TNF-αandIL-8mRNA.TheproductionofIL-1β,TNF-αandIL-8andtheexpressionofmRNAweredown-regulatedbypyrrolidinedithiocarbamate(PDTC).ThisstudydemonstratedthatM.penetransLAMPscaninducetheproductionofproinflammatorycytokinesinhumanmonocyticcellsinvitro,thussuggestingthatitmaybeanimportantetiologicalfactor.
出版日期
2006年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
