细胞学薄层液基涂片技术在免疫组化染色中的应用

摘要目的探讨细胞学薄层液基涂片技术在免疫组化中的应用。方法选用有对比意义的二组病例。计有腺癌性胸腹水液基涂片74例。小细胞未分化痰涂片40例。采用免疫组化二代二步法进行染色。结果腺癌性胸腹水薄层液基涂片中腺癌细胞对CEA抗体显阴性，间皮细胞对mesothelin（间皮素）抗体显阳性；小细胞未分化痰涂片中小细胞未分化癌细胞对CHA抗体显阳性。淋巴细胞对LCA抗体显阳性。结论免疫组化的抗原抗体反应是具有高度特异性和敏感性的生物化学反应，对特定抗原识别力强。在细胞、亚细胞水平检测抗原分子是其他生物技术难以达到和替代的。在细胞学上具有广阔的应用前景。

• 作者: 赵乐韩玲闫振宇
• 出处: 《中国健康月刊》 2011年12期
• 关键词: 细胞学 薄层液基涂片 染色 方法Abstract objective to study thin-layer liquid-based cytology smear technique and its application in Immunohistochemistry. Method the use of comparative significance of two groups of cases. There were 74 cases of adenocarcinoma in pleural fluid-based smear. 40 cases of small cell resolving phlegm and smear. Second generation using immunohistochemical two-step dye. Results adenocarcinoma in pleural effusion and ascites in a thin layer of liquid based smears adenocarcinoma cells on CEA antibody-negative Mesothelial cells to mesothelin (mesothelin) antibody-positive small small cell undifferentiated carcinoma cell and resolving phlegm smear on CHA antibody-positive. On LCA antibody-positive lymphocytes. Conclusion Immunohistochemical Antigen-antibody reaction is a high degree of specificity and sensitivity of biochemical reactions to a specific antigen recognition and strong. In cells subcellular level detection Antigen molecule is difficult for other biotechnology and alte
• 分类: [医药卫生][公共卫生与预防医学]